| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218535 |
| Chromosome: | chromosome 9 |
| Location: | 6530290 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g407750 | CGL130 | Conserved in the Green Lineage | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCCACTAAACTCTCGAAACCAGCGTATT |
| Internal bar code: | GTATTAGAAGAGCATATTCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1178 |
| LEAP-Seq percent confirming: | 99.2481 |
| LEAP-Seq n confirming: | 528 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTCAATGCTGCTGTTGGA |
| Suggested primer 2: | CTTAGACCACTCGGCCACAT |