Insertion junction: LMJ.RY0402.218543_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.218543
Chromosome:chromosome 17
Location:6123236
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g741500 KIL7 Putative kinesin-like motor protein; (1 of 1) 3.4.21.72//3.6.4.4 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease // Plus-end-directed kinesin ATPase / Kinesin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGCAACGCCTCACAACAACGATTTCCGA
Internal bar code:TAGGTATTTCTCCCTTGTACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:682
LEAP-Seq percent confirming:98.8817
LEAP-Seq n confirming:2299
LEAP-Seq n nonconfirming:26
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACGAGATCGTTCAAGATGGG
Suggested primer 2:CGCCAGGTTGGTGTACTTTT