| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218546 |
| Chromosome: | chromosome 6 |
| Location: | 2619057 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269752 | TRXm,TRXM1,TRX15 | (1 of 1) IPR000104//IPR005746//IPR012336//IPR013766 - Antifreeze protein, type I // Thioredoxin // Thioredoxin-like fold // Thioredoxin domain; Thioredoxin m, chloroplastic | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTTGCATGACGCAGGCACGGCTCGGCTG |
| Internal bar code: | CTCTAGGCGCAAGCACCACTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 947 |
| LEAP-Seq percent confirming: | 99.6723 |
| LEAP-Seq n confirming: | 11558 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCTCCCTTCCCTTGTTT |
| Suggested primer 2: | CTACATGCGCACGACTGATT |