| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218552 |
| Chromosome: | chromosome 6 |
| Location: | 5682827 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g286450 | (1 of 1) PTHR22847//PTHR22847:SF489 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGGGCATATTGCTGTTGGTGAGGTGTTG |
| Internal bar code: | TAGACACATTGTTTATGCTCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 563 |
| LEAP-Seq percent confirming: | 70.3008 |
| LEAP-Seq n confirming: | 187 |
| LEAP-Seq n nonconfirming: | 79 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACGCATGTATGGTAGTCG |
| Suggested primer 2: | ATGAACTTCCGCGTAACCAC |