| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218554 |
| Chromosome: | chromosome_7 |
| Location: | 623419 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g316992 | antisense | intron|outside_mRNA |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCATAAGTTTTATTGCTTGCATCTGTCGC |
| Internal bar code: | ACGCGTACTCCCTCGCGTGTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 95.1057 |
| LEAP-Seq n confirming: | 855 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAATCCATCCTCGCTCTC |
| Suggested primer 2: | GACCACGATGAAGTGTCCCT |