Insertion junction: LMJ.RY0402.218569_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.218569
Chromosome:chromosome 7
Location:4417002
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g340850 CYP743A2,CYP19 (1 of 3) 1.14.14.1//5.3.99.5 - Unspecific monooxygenase / Xenobiotic monooxygenase // Thromboxane-A synthase / Thromboxane synthetase; Cytochrome P450, CYP197 superfamily CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTGTCAGCCCCTCGCCCGTGCTCTTGTC
Internal bar code:TTGGCGTACTGCTCAAAATTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:576
LEAP-Seq percent confirming:99.3377
LEAP-Seq n confirming:150
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGATGCTCAAGACCGTG
Suggested primer 2:GCTTGCTCAGGAACAAGTCC