Insertion junction: LMJ.RY0402.218592_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.218592
Chromosome:chromosome 2
Location:3270778
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGGAAGCTGCGCGTGGGCCGCGGCCTGCT
Internal bar code:TGGGAGTTCGTCTTATACTCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:228
LEAP-Seq percent confirming:99.4798
LEAP-Seq n confirming:1530
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:AACCGTGGGTACATAGCTCG
Suggested primer 2:TTCACATCGGGATTCCTAGC