Insertion junction: LMJ.RY0402.218592_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.218592
Chromosome:chromosome 2
Location:3270841
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g095081 (1 of 1) PF09295 - ChAPs (Chs5p-Arf1p-binding proteins) (ChAPs) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCAATACAATGCGCATCCCTCGAGCGCGCT
Internal bar code:CTAATGGCGCTCTATATCGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:104
LEAP-Seq percent confirming:98.8466
LEAP-Seq n confirming:857
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TTCACATCGGGATTCCTAGC
Suggested primer 2:AACCGTGGGTACATAGCTCG