| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218593 |
| Chromosome: | chromosome 2 |
| Location: | 7056530 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g147301 | (1 of 33) IPR013763 - Cyclin-like | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGCGATGGGAGTCATGGCACACGTGC |
| Internal bar code: | ATGTTGATCCGACCGAGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 970 |
| LEAP-Seq percent confirming: | 98.8498 |
| LEAP-Seq n confirming: | 4211 |
| LEAP-Seq n nonconfirming: | 49 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAACTGAATGGCTGCTGA |
| Suggested primer 2: | AGCTGTACCCCAACAGGATG |