Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.218596 |
Chromosome: | chromosome_7 |
Location: | 5414595 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre07.g350350 | FAP217 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTGGGCCGAGGATAATGTTGAACTGTAGA |
Internal bar code: | CATTGGGTTACTAAGAGCTAGG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 696 |
LEAP-Seq percent confirming: | 96.9757 |
LEAP-Seq n confirming: | 2950 |
LEAP-Seq n nonconfirming: | 92 |
LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTGTCATAACCACGCGACAT |
Suggested primer 2: | CCAGTACTCCTCCTGCTTGC |