| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218598 |
| Chromosome: | chromosome 9 |
| Location: | 863374 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401650 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGGGGTCGCGAGTTGTCTGCGTGCAG |
| Internal bar code: | GGGGAGCTTAGGTCCTTTTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 959 |
| LEAP-Seq percent confirming: | 99.28 |
| LEAP-Seq n confirming: | 4964 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATTTGATGCATAGGGCGAG |
| Suggested primer 2: | TGAAGCTGAACCTGTTGTCG |