| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218601 |
| Chromosome: | chromosome 9 |
| Location: | 754118 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402400 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAGCCCATGCTATTGTACGGTGCAATACA |
| Internal bar code: | AAACACACTCCATAGTTGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 414 |
| LEAP-Seq percent confirming: | 99.1909 |
| LEAP-Seq n confirming: | 1226 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGACTCGTGGAATGAGCAG |
| Suggested primer 2: | GGAGGAATGGTAGGCGAAAT |