| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218615 |
| Chromosome: | chromosome 6 |
| Location: | 1438309 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g259200 | EFS2,EFS1,SELB,SELB1 | Selenocysteine-specific elongation factor EF-Sec; (1 of 1) PTHR23115:SF91 - SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGATCACTGCACACTTGTTGCTTGTGCCTG |
| Internal bar code: | GAATCATAGCAGAAGCCACGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 381 |
| LEAP-Seq percent confirming: | 99.3935 |
| LEAP-Seq n confirming: | 5900 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGGGGGAGTGTTGTTAGTA |
| Suggested primer 2: | CTGGAGAGGCTGTTACCTGG |