| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218642 |
| Chromosome: | chromosome 6 |
| Location: | 6906414 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g295826 | (1 of 1) IPR002048//IPR007065 - EF-hand domain // HPP | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGTGTGCCTGTCCTGAACACTGCCCGC |
| Internal bar code: | ACTTTCCCCCCTTCGATTTTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 336 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCCCCACTCCTCCTACTC |
| Suggested primer 2: | GTTGATCCTAACCGGGGAAT |