| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218664 |
| Chromosome: | chromosome 16 |
| Location: | 677051 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g691600 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTCACGTTCGACACCCAACTACTCCCCA |
| Internal bar code: | AATGTTTCAGGGGCAACGTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 423 |
| LEAP-Seq percent confirming: | 99.7067 |
| LEAP-Seq n confirming: | 2380 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTCAACCAGTCACCCTTTT |
| Suggested primer 2: | GCACAGGCACATACACCATC |