Insertion junction: LMJ.RY0402.218675_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.218675
Chromosome:chromosome_13
Location:1499427
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre13.g572700 FLA15,FAP66,IFT144 Intraflagellar transport protein 144 antisense intron

Insertion site details

Flanking sequence (orientation from cassette outwards):CAGGGAAACGTGGCGGGGTACGACAGTGAG
Internal bar code:TTCAGTCGTTAGTGGTGCAATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1152
LEAP-Seq percent confirming:99.4891
LEAP-Seq n confirming:10320
LEAP-Seq n nonconfirming:53
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGCTGGTTAGTGGTCGT
Suggested primer 2:CAACGAGATGGACACGTTTG