Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.218675 |
Chromosome: | chromosome_13 |
Location: | 1499427 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g572700 | FLA15,FAP66,IFT144 | Intraflagellar transport protein 144 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAGGGAAACGTGGCGGGGTACGACAGTGAG |
Internal bar code: | TTCAGTCGTTAGTGGTGCAATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1152 |
LEAP-Seq percent confirming: | 99.4891 |
LEAP-Seq n confirming: | 10320 |
LEAP-Seq n nonconfirming: | 53 |
LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AGCAGCTGGTTAGTGGTCGT |
Suggested primer 2: | CAACGAGATGGACACGTTTG |