| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218677 |
| Chromosome: | chromosome 12 |
| Location: | 8575164 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g547350 | CYG31 | Adenylate/guanylate cyclase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACTTGTCAACACGCTCCCTCACATCA |
| Internal bar code: | GATTCATATTAGATCTCAGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 245 |
| LEAP-Seq percent confirming: | 99.7423 |
| LEAP-Seq n confirming: | 1161 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATGCACCTCATGATGAAT |
| Suggested primer 2: | AAGCTCCGGCCTCTTACTTC |