| Insertion cassette: | CIB1 | 
| Side of cassette: | 3' | 
| Strand: | + | 
| Strain: | LMJ.RY0402.218685 | 
| Chromosome: | chromosome 7 | 
| Location: | 5849236 | 
| Confidence (%): | 58 | 
| Locus systematic id | Locus common name | Defline | Feature | 
|---|---|---|---|
| intergenic | 
| Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATAGGTTGGGGGCAACAGGCACAGCGG | 
| Internal bar code: | GAGTATCGCTCCAGGCGGGTCG | 
| Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 236 | 
| LEAP-Seq percent confirming: | 50.0 | 
| LEAP-Seq n confirming: | 2 | 
| LEAP-Seq n nonconfirming: | 2 | 
| LEAP-Seq n unique pos: | 2 | 
| Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGAAAGCAAGCAGGTC | 
| Suggested primer 2: | CTAGGCCCGTACGACCTGTA |