| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218696 |
| Chromosome: | chromosome_2 |
| Location: | 4964714 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g103950 | FAP100 | Flagellar Associated Protein | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGGTTCAACGTTAACCACATACACCACCCT |
| Internal bar code: | CGCGACTTCAGCTCATGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 275 |
| LEAP-Seq percent confirming: | 95.7346 |
| LEAP-Seq n confirming: | 808 |
| LEAP-Seq n nonconfirming: | 36 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAGGGTTGTGGATGTGTG |
| Suggested primer 2: | CCAGGTACCCGTGCCTAGTA |