| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218738 |
| Chromosome: | chromosome 7 |
| Location: | 908386 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318950 | (1 of 1) IPR000008//IPR000104//IPR003072 - C2 domain // Antifreeze protein, type I // Orphan nuclear receptor, NOR1 type | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTTCGTGTGTGGTCAGGTGTGCGATTG |
| Internal bar code: | AATTTTCAAACTGTATTCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 976 |
| LEAP-Seq percent confirming: | 99.6056 |
| LEAP-Seq n confirming: | 5809 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTTGTGATACTCCCTGGC |
| Suggested primer 2: | TAGCGTCTCGCCTGTATGTG |