| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218739 |
| Chromosome: | chromosome_3 |
| Location: | 7602330 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g204601 | FAP278,CPK3 | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCGGCTGCACGTGGATGGATGCATGCTCG |
| Internal bar code: | GATCTGTTCCAGTTGCGCTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 276 |
| LEAP-Seq percent confirming: | 99.6483 |
| LEAP-Seq n confirming: | 4533 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAAAGCACCCTTACCCAAC |
| Suggested primer 2: | AAGGAGTATGGGTGTGCTGG |