| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218739 |
| Chromosome: | chromosome 16 |
| Location: | 5911734 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677100 | (1 of 781) IPR000104 - Antifreeze protein, type I | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGATACATCCACAAGGAACAATCAAGTGG |
| Internal bar code: | ACGGTCCGCGCCGAAGCTCCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 922 |
| LEAP-Seq percent confirming: | 97.6197 |
| LEAP-Seq n confirming: | 3773 |
| LEAP-Seq n nonconfirming: | 92 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTGTGAGTGTCCAATGCC |
| Suggested primer 2: | GAGTGAGCGCGCTAGTTTCT |