| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218778 |
| Chromosome: | chromosome 6 |
| Location: | 2504565 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269050 | TIC62,YCF39-2 | (1 of 2) PTHR14194:SF71 - PRESTALK A DIFFERENTIATION PROTEIN A; Putative dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATTGCAAGTGACCTCACCAAGCATGCTC |
| Internal bar code: | AAGGGGTGGCGCCTAGCCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 566 |
| LEAP-Seq percent confirming: | 99.62 |
| LEAP-Seq n confirming: | 10487 |
| LEAP-Seq n nonconfirming: | 40 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTCGCTCTGCTACCTCTC |
| Suggested primer 2: | GTAATGAGTGCGGACTGGGT |