Insertion junction: LMJ.RY0402.218788_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.218788
Chromosome:chromosome_9
Location:7073659
Confidence (%):95
Locus systematic id Locus common name Defline Orientation Feature
Cre09.g411525 antisense CDS

Insertion site details

Flanking sequence (orientation from cassette outwards):TGTGGTCGGGCCTGTGAAGACAGCGAGTGC
Internal bar code:TACTCCCCCTGACAACGTCAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:404
LEAP-Seq percent confirming:51.3889
LEAP-Seq n confirming:2627
LEAP-Seq n nonconfirming:2485
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACGCAC
Suggested primer 2:TGCTGGAGCTAACCCCTAGA