| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218788 |
| Chromosome: | chromosome_9 |
| Location: | 7073667 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g411525 | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GAGGTCTGCCGCGGTGCGGGTGAGCGCCGG |
| Internal bar code: | CGAAGCGAATTGCGTTCCCAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 472 |
| LEAP-Seq percent confirming: | 98.958 |
| LEAP-Seq n confirming: | 32858 |
| LEAP-Seq n nonconfirming: | 346 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACACACACACACACGCAC |
| Suggested primer 2: | TGCTGGAGCTAACCCCTAGA |