Insertion junction: LMJ.RY0402.218788_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.218788
Chromosome:chromosome 11
Location:2625143
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre11.g476376 FAP221,Pcdp1,RPC82 Flagellar Associated Protein 221; (1 of 1) PTHR23053//PTHR23053:SF21 - DLEC1 DELETED IN LUNG AND ESOPHAGEAL CANCER 1 // SUBFAMILY NOT NAMED 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGACTTGCACATGTAACTGTACGACGGCT
Internal bar code:CTTGGAAAGAGGGTCCGCCGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:957
LEAP-Seq percent confirming:99.7449
LEAP-Seq n confirming:391
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CAATTCGCTCTGCACCTGTA
Suggested primer 2:TCAGTGCTGGACTTCGTTTG