| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218810 |
| Chromosome: | chromosome 5 |
| Location: | 883235 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g247550 | GST7 | (1 of 1) PTHR11260:SF220 - GST-CONTAINING FLYWCH ZINC-FINGER PROTEIN; Glutathione S-transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCGTAAGCCAACTACTGCCAGCAGTGCC |
| Internal bar code: | AACTGGCGCCGATTTAGTTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 474 |
| LEAP-Seq percent confirming: | 99.6883 |
| LEAP-Seq n confirming: | 5757 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTTGTGAGTTCGTGCGTGT |
| Suggested primer 2: | GGAGGCAGAATCGTTCAGAG |