| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218812 |
| Chromosome: | chromosome 7 |
| Location: | 6416032 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g358050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCGGAACGGTGGAAGAGGGCTAGCACTG |
| Internal bar code: | TTCCGTCCACCGGATTATAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 99.0559 |
| LEAP-Seq n confirming: | 9968 |
| LEAP-Seq n nonconfirming: | 95 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGTGAGGAGACCGAAAAG |
| Suggested primer 2: | CCGTGACAGGTACGTTGATG |