Insertion junction: LMJ.RY0402.218847_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.218847
Chromosome:chromosome 1
Location:3932775
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre01.g025650 (1 of 1) IPR013286 - Annexin, type VII 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGATAATGCCAACAAATGACGTCGTCCTA
Internal bar code:TTTTTCGACAACGGCCAGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:996
LEAP-Seq percent confirming:99.6479
LEAP-Seq n confirming:8773
LEAP-Seq n nonconfirming:31
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGAACCTTTCTTCAACGC
Suggested primer 2:TTGAACTGGGATGTTAGCCC