Insertion junction: LMJ.RY0402.218849_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.218849
Chromosome:chromosome 13
Location:4739693
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre13.g604850 (1 of 1) IPR026126 - BRISC and BRCA1-A complex member 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGAGCGACGGCGCGGTGCGGGCGCGGCGT
Internal bar code:GCACCAACCAGATGGGTAACGA

Confirmation - LEAP-Seq

LEAP-Seq distance:655
LEAP-Seq percent confirming:37.931
LEAP-Seq n confirming:869
LEAP-Seq n nonconfirming:1422
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGCTTACTCCACACGACA
Suggested primer 2:TTCAAAATTCAAGCGTGCTG