| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218852 |
| Chromosome: | chromosome 1 |
| Location: | 1696231 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g008950 | (1 of 1) IPR024930 - Skp domain | 5'UTR|outside_mRNA | |
| Cre01.g008976 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGCGATTACGTGGGGGCTTCGCAGCAGA |
| Internal bar code: | AACCCGGGAGGATCCGGGGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 363 |
| LEAP-Seq percent confirming: | 99.6166 |
| LEAP-Seq n confirming: | 1559 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTCCGACAGGAAACTCAA |
| Suggested primer 2: | TGCTTGAAAGAACGCAACAC |