| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218870 |
| Chromosome: | chromosome 5 |
| Location: | 2246984 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234662 | (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTCGGCGCTGTTTTCGGGGCGAAAGAAG |
| Internal bar code: | ATGCTCGGTCCAGAGTGGGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1034 |
| LEAP-Seq percent confirming: | 99.6118 |
| LEAP-Seq n confirming: | 1796 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACAGAGTGGCCTGCAGAAT |
| Suggested primer 2: | CCGAATCCTTACAGCCATGT |