| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218880 |
| Chromosome: | chromosome 12 |
| Location: | 3493248 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501717 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCGTAGAAGATGCCTGCGCACACACCGAA |
| Internal bar code: | GGGTTACACTCTTTGTGACCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 676 |
| LEAP-Seq percent confirming: | 98.9015 |
| LEAP-Seq n confirming: | 67072 |
| LEAP-Seq n nonconfirming: | 745 |
| LEAP-Seq n unique pos: | 177 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCGACTGTACTGACCCCT |
| Suggested primer 2: | CATCGCCTTTCACTGCTACA |