| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218899 |
| Chromosome: | chromosome 11 |
| Location: | 3646257 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482300 | FAP305,MOT17 | Motility 17; (1 of 1) PTHR22706:SF0 - SPERMATOGENESIS-ASSOCIATED PROTEIN 17 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGCACACCTGATTGTACCACGGCGTATT |
| Internal bar code: | CGGACGGCGGGTGCGGTTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 110 |
| LEAP-Seq percent confirming: | 92.7007 |
| LEAP-Seq n confirming: | 254 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGGGTTGACCATGCCTC |
| Suggested primer 2: | TTGGTGTGGACAAGAATGGA |