| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.218899 |
| Chromosome: | chromosome 11 |
| Location: | 3646269 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482300 | FAP305,MOT17 | Motility 17; (1 of 1) PTHR22706:SF0 - SPERMATOGENESIS-ASSOCIATED PROTEIN 17 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGGGTGGGATGGTGTACGATGATGGCGGA |
| Internal bar code: | ATTTTGTATGCTGCTTTACTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 166 |
| LEAP-Seq percent confirming: | 98.2716 |
| LEAP-Seq n confirming: | 398 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTAGGGTTGACCATGCCTC |
| Suggested primer 2: | TTGGTGTGGACAAGAATGGA |