| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.218901 |
| Chromosome: | chromosome 3 |
| Location: | 6546234 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195600 | (1 of 1) 3.2.1.28 - Alpha,alpha-trehalase / Trehalase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCCCGAATGCACGTGCCTCCTCCTCTGGC |
| Internal bar code: | AACGGGTGTAGGGTTGGAGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 305 |
| LEAP-Seq percent confirming: | 99.6343 |
| LEAP-Seq n confirming: | 3269 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTACGGCAGGATACCGAA |
| Suggested primer 2: | TGACCCAGTAGGAGTCCCAG |