| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218918 |
| Chromosome: | chromosome 9 |
| Location: | 4781900 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g397327 | (1 of 1) PTHR11139:SF68 - DNA-DEPENDENT PROTEIN KINASE CATALYTIC SUBUNIT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCATCCTCCTCGCCGCTTCTCTCGCCGCCT |
| Internal bar code: | TTATAGTAGGTTTCAACTCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 79 |
| LEAP-Seq percent confirming: | 71.5026 |
| LEAP-Seq n confirming: | 138 |
| LEAP-Seq n nonconfirming: | 55 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGACTGCAAATCGCATTA |
| Suggested primer 2: | AGGAGGGAGAGGATGAGGAC |