| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218951 |
| Chromosome: | chromosome 12 |
| Location: | 5632376 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g532200 | AOA2 | Putative alcohol O-acetyltransferase; (1 of 4) 2.3.1.84 - Alcohol O-acetyltransferase / AATASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAATCGTGACCGGCCGGCTGTCTCCTGG |
| Internal bar code: | TTGATTCAGAGCAGCCAGGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 901 |
| LEAP-Seq percent confirming: | 92.2676 |
| LEAP-Seq n confirming: | 2303 |
| LEAP-Seq n nonconfirming: | 193 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGCACAACAACTGCAAGGA |
| Suggested primer 2: | CCCTATTCAGCGCTTTCTTG |