| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218969 |
| Chromosome: | chromosome 12 |
| Location: | 7146517 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g559000 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCTGCGCCTCCAGGGCGCAGGCTTTGTG |
| Internal bar code: | GCCGGACGGGTAGGTAATCCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 99.7399 |
| LEAP-Seq n confirming: | 3451 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGTAAACCCGAAACACTGC |
| Suggested primer 2: | CATAAGCGCGCGTACATAGA |