| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.218975 |
| Chromosome: | chromosome 16 |
| Location: | 2111614 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g657850 | SEC63 | (1 of 1) K09540 - translocation protein SEC63 (SEC63, DNAJC23); ER-targeted preprotein translocase subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGGTAGTAGCGTGACTCGCAGCACGGGG |
| Internal bar code: | TAACGCCACTACTGGAAGCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 772 |
| LEAP-Seq percent confirming: | 96.821 |
| LEAP-Seq n confirming: | 3137 |
| LEAP-Seq n nonconfirming: | 103 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTAGGCCTTGGTGATGT |
| Suggested primer 2: | CACAACATCAACAGGCAACC |