Insertion junction: LMJ.RY0402.218980_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.218980
Chromosome:chromosome 8
Location:1988595
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre08.g367950 FAP323 (1 of 12) IPR000595//IPR018490 - Cyclic nucleotide-binding domain // Cyclic nucleotide-binding-like; Cyclic nucleotide-monophosphate binding domain Flagellar Associated Protein 323 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAGCCAGGCTCCCATGTGACCTGCAGCT
Internal bar code:ACGACGCCGGGCTCCGGCGCTG

Confirmation - LEAP-Seq

LEAP-Seq distance:0
LEAP-Seq percent confirming:0.0677966
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:4422
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:ACCCTTCATGGCTACGTGTC
Suggested primer 2:GATTTCGCTTGTTGAGAGGC