Insertion junction: LMJ.RY0402.218980_3

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.218980
Chromosome:chromosome 8
Location:4705720
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre08.g384000 CYP26,CYP771A1 (1 of 3) IPR001128//IPR002397//IPR002401//IPR002403 - Cytochrome P450 // Cytochrome P450, B-class // Cytochrome P450, E-class, group I // Cytochrome P450, E-class, group IV; Cytochrome P450, CYP197 superfamily gene_edge/mRNA_edge/3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTTTCCGAGCTTTACATACACCACACCAC
Internal bar code:AAAGGTGTGCATATCGGTCCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:843
LEAP-Seq percent confirming:99.3162
LEAP-Seq n confirming:11620
LEAP-Seq n nonconfirming:80
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGCCTGGTTAGTTTGTGG
Suggested primer 2:AGTCCATCGTCATCACCTCC