| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218980 |
| Chromosome: | chromosome_8 |
| Location: | 4705720 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g384000 | CYP771A1,CYP26 | Cytochrome P450, CYP197 superfamily | antisense | gene_edge/mRNA_edge/3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTTTCCGAGCTTTACATACACCACACCAC |
| Internal bar code: | AAAGGTGTGCATATCGGTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 99.3162 |
| LEAP-Seq n confirming: | 11620 |
| LEAP-Seq n nonconfirming: | 80 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCGCCTGGTTAGTTTGTGG |
| Suggested primer 2: | AGTCCATCGTCATCACCTCC |