| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.218999 |
| Chromosome: | chromosome 12 |
| Location: | 5568396 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531600 | EIF5Bd | (1 of 1) K13690 - alpha-1,3-mannosyltransferase [EC:2.4.1.-] Man a1-3 Man (CMT1); putative translation initiation factor eIF-2 beta chain | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCCATAGCTTAAGCATGTAACTGTGGGC |
| Internal bar code: | CCGACTACCGACGAGCGTAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 908 |
| LEAP-Seq percent confirming: | 99.763 |
| LEAP-Seq n confirming: | 3367 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCGAGGTGTACTGCTGCT |
| Suggested primer 2: | GTGAAGCAGAGGATAAGGCG |