| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219010 |
| Chromosome: | chromosome 2 |
| Location: | 8486529 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g141806 | PTK16 | (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGATTTTGGCGGTTAGGGCGAAGAGCGCAG |
| Internal bar code: | CCGTGGGTCGTCGGTTCGTGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 99.6641 |
| LEAP-Seq n confirming: | 11276 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCTTTTGCACTTTTGCACG |
| Suggested primer 2: | CAAGTGTCCTCGCATCAAGA |