Insertion junction: LMJ.RY0402.219031_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.219031
Chromosome:chromosome 12
Location:33654
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre12.g483500 (1 of 1) PF06101//PF12624//PF16908//PF16910 - Plant protein of unknown function (DUF946) (DUF946) // N-terminal region of Chorein or VPS13 (Chorein_N) // Vacuolar sorting-associated protein 13, N-terminal (VPS13) // Repeating coiled region of VPS13 (VPS13_mid_rpt) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGGGTGCATGAAGGAACTGGGTCTCGCGT
Internal bar code:TCCCTGCCGATCGGTTCGCCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:417
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GGTTCAGCTTCAGGTTCAGC
Suggested primer 2:GGGTAGGGACAGTCCACAGA