| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.219035 |
| Chromosome: | chromosome 10 |
| Location: | 183059 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g418976 | 3'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATCACGATTGCCACCCAGGCACCCAGGCT |
| Internal bar code: | CCTGCACCCAGCTGGGATGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 349 |
| LEAP-Seq percent confirming: | 74.0088 |
| LEAP-Seq n confirming: | 336 |
| LEAP-Seq n nonconfirming: | 118 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAACCTTTGTCCTTCTGGG |
| Suggested primer 2: | ACATGGTTTGGCTAAGTGCC |