| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219037 |
| Chromosome: | chromosome 13 |
| Location: | 1533962 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572850 | FBB17 | (1 of 6) PF00246 - Zinc carboxypeptidase (Peptidase_M14); Flagellar/basal body protein 17 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAAAGCCACTTGCCCACTGCAACCGCTGG |
| Internal bar code: | ATTGGCATCGAGAGCTGATCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 438 |
| LEAP-Seq percent confirming: | 97.1429 |
| LEAP-Seq n confirming: | 918 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGAGGGAGCTGAAGGTAGC |
| Suggested primer 2: | TCAGAACCTCTGGAACAGGG |