| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.219050 |
| Chromosome: | chromosome 16 |
| Location: | 2572995 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661450 | HTV3 | (1 of 35) K11253 - histone H3 (H3); Histone H3 variant | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGATGGCTGGGGGCTTGGCTGGGGGCG |
| Internal bar code: | GCTAGAACAGTCCAATAAGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 935 |
| LEAP-Seq percent confirming: | 98.4939 |
| LEAP-Seq n confirming: | 2485 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTGCTCAGACCGTTGTCA |
| Suggested primer 2: | CATGGGCTCTGAAGATGGAT |