| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.219096 |
| Chromosome: | chromosome 9 |
| Location: | 7110696 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411850 | (1 of 2) PTHR23130:SF61 - DEFENSE PROTEIN L(2)34FC | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTTTACTATGCGGCTCTTGACTGTACC |
| Internal bar code: | TCTCGATGTTACTTGTTGCCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.2941 |
| LEAP-Seq n confirming: | 4642 |
| LEAP-Seq n nonconfirming: | 33 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCACCTCTAATGGCTCCAG |
| Suggested primer 2: | GAGACCATGATTGGCTCGAT |