| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.219102 |
| Chromosome: | chromosome 16 |
| Location: | 2523619 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g661100 | MAPK7 | (1 of 1) K08829 - male germ cell-associated kinase [EC:2.7.11.22] (MAK); Mitogen-Activated Protein Kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCGTCCATTGCCCTCCTCCCAGCCCC |
| Internal bar code: | AATCTGCGCAATTCCAGGATCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 457 |
| LEAP-Seq percent confirming: | 99.5542 |
| LEAP-Seq n confirming: | 4243 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGGACTCCAGCTCAACCT |
| Suggested primer 2: | GTGAGGAGTGAAGTGCACGA |